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This book is a compilation of cytogenetic and molecular cytogenetic techniques that are routinely performed in a molecular cytogenetic laboratory. It provides a summary of chromosomal disorders and mechanisms, along with pictures and details of laboratory procedures. Due to the simplicity of the language used, the principles and techniques discussed here are easily understandable. The book also details modern techniques, which will be of interest for geneticists, academicians, scientists, and clinical geneticists aspiring to establish a molecular cytogenetic lab. It also serves to help geneticists understand each protocol as it is written in a self-explanatory manner for standardizing techniques in their laboratory.
Plant cytogenetics has progressed at a rapid rate since the publication of the first edition. Plant Cytogenetics, Second Edition presents an up-to-date review of cytogenetics. It covers the latest in the various classical and modern techniques in the handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulation. It includes new chapters on extra chromosomal inheritance and the mode of reproduction in plants, paricularly apomixis, as well as new sections on the molecular basis of heredity, genomic in situ hybridization, and the classical and molecular methods of genome analysis. The author also elaborates on the cytogenetic basis of somaclonal variation generated through cell and tissue culture.
In the summer of 1989, one of us (SLG), along with his mentor, Dorothy Warb- ton, attended the Tenth International Workshop on Human Gene Mapping. The me- ing was held at Yale University in celebration of the first such event, which also took place there. This meeting was not open to the general public; one had to have contributed to mapping a gene to be permitted to attend. The posters, of course, were therefore all related to gene mapping, and many were covered with pretty, colorful pictures of a novel, fluorescent application of an old technology, in situ hybridization. Walking through the room, Dorothy remarked that, because of this new FISH technique, ch- mosomes, which had become yeste...
In preparing the new completely revised edition of this glossary, which in the meantime has been translated into Russian and Polish, we have attempt ed to include the most important new terms and to revise the text in those cases where new data demanded it*. As a result about fifty percent of the text is completely rewritten. Once more we have tried to provide material suitable and usable both for students and research workers. Accordingly, depending upon our evaluation, some terms have been simply defined, others have been described at some length even to the extent of providing experi mental data. Wherever possible, synonymy and redundancy have been pointed out, and in the interest of hist...
Human Cytogenetics: Constitutional Analysis covers all basic aspects of human cytogenetic study other than malignancies and abnormalities. They are covered in a separate volume. Since the publication of the 2nd edition in 1992, there have been major advances in technology and the emphasis of this new edition is on the spectrum of technologies available to conventional and molecular cytogenetics. Perhaps the largest new development has been the transition of fluorescence in situ hybridization to an essential tool for all cytogenetecists and consequently its use in chromosome analysis is covered in detail. Another important new technology to be described in detail is computerised image analysi...
There are about 300-315 thousand species of plants, of which the great majority, some 260-290 thousand, are seed plants. Green plants provide a substantial proportion of the world's molecular oxygen and are the basis of most of Earth's Ecologies, especially on land. Plants that produce grains, fruits and vegetables form humankind's basic foodstuffs, and have been domesticated for millennia. Plants play many roles in culture. They are used as ornaments and, until recently and in great variety, they have served as the source of most medicines and drugs. The scientific study of plants is known as botany, a branch of biology. Plant Cytogenetics, Breeding and Evolution Plant Cytogenetics comprises a topic of broad interest and increasing importance in plant science. In keeping with the exciting advances in plant genetics and genomics, we believe that a comprehensive and up-to-date reference on Plant Cytogenetics would be of great interest and value for researchers, instructors, and students with interests in genetics, plant biology, and plant genomics.
The history of science is mostly written retrospec tively, a generation or two after the actual events being discussed. Science historians are now analyzing and evaluating the origins of evolutionary and genetical theory in the nineteenth century and a sort of "Darwin industry" seems to have grown up. A history of mammalian cytogenetics by one of the main participants is, hence, a very welcome change, since it has a vividness, an immediacy and a personal flavor which these scholarly tomes and the official biog raphies of scientists mostly lack. The life of the author, Chinese-born, T. C. Hsu, has been a romantic and color ful one, and he is himself a unique personality, so that his book is a very unusual blend of reminiscences, history of his special field (which has transformed human genetics) and wise comments on the mistakes made along the way. The best qualities of a very fine Chinese mind have contributed to Dr. Hsu's career, including this book. Those qualities (which seem to me especially Chinese) include a kind of transparent honesty, a very direct em pirical approach to problems and superb technical ability.
An introductory discussion of basic chromosome structure and function preceeds the main text on the application of cytogenetic approaches to the analysis of the manipulation of both the genetic make-up and the genetic transmission system of plant breeding material. Analysis using light and electron microscopy, segregations and molecular techniques, yields information for assessing the material before and after manipulation. Much attention is given to quantitative methods. Manipulation not only involves the construction of specific genotypes, but also chromosomal transmission systems. Although analysis and manipulation in the somatic cycle are considered, the focus is on the generative cycle, with emphasis on analysis and subsequent segregation of specifically constructed material. The book is intended for plant breeders and other scientists interested in the analysis and manipulation of breeding material at the chromosomal level. Comparisons with molecular and cell biological approaches are made, and the potential of the various methods is evaluated.
For Degree level Students
A few members of the Gossypium (cotton) genus are cultivated for the production of elongated single-celled fibers valued worldwide at about $20 billion annually at the farm gate, and which sustain one of the world’s largest industries (textiles) with an annual worldwide economic impact of about $500 billion. In a number of ways, cotton production and the textile industry are closely tied to petrochemical usage. At a practical level, cotton genomics offers both bio-based carbon sequestration alternatives to petrochemical use, and improved sustainability of crop production. The Gossypium (cotton) also genus presents novel opportunities to advance our understanding of the natural world and it...