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This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins; live-imaging centriole amplification in mouse brain multiciliated cells; biophysical properties of cilia motility; and mucociliary transport device construction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cilia: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
This book is a thorough guide to dialysis that will assist in the effective treatment of patients. Current knowledge on all aspects of dialysis, including the most recent advances, is presented and the various techniques used in dialysis patients are described step by step. Both hemodialysis and peritoneal dialysis are fully considered. The coverage of hemodialysis explains the procedure and its indications and provides guidance on challenging topics such as vascular access, response to acute and chronic complications, and on-line hemodiafiltration. The discussion of peritoneal dialysis includes explanation of peritoneal physiology, the principles of prescription, and the treatment of peritonitis and exit site infection. A summary of updated dialysis-related guidelines is included at the end of the book. The lucid text is supported by more than 200 full-color illustrations that clarify anatomy, vascular/peritoneal access, and dialysis procedures and techniques. The Essentials of Clinical Dialysis will be of value for all who are involved in the management of dialysis patients and will be a helpful guide for training of physicians and healthcare associates in the dialysis center.
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In 1994, the expert knowledge of Ram Gokal and Karl D. Nolph, the two foremost figures in the field of peritoneal dialysis, was combined to produce the first edition of the Textbook of Peritoneal Dialysis. The work quickly became recognised as the `gold standard' for those working in the field. Since its conception, however, our understanding of peritoneal dialysis related physiology, kinetics and clinical outcomes, as well as the concepts of intraperitoneal chemotherapy, has increased sufficiently to make an updated and completely revised edition of the work necessary. An expansion of the editorial team by fellow-experts Ramesh Khanna and Raymond Krediet enabled an even more comprehensive approach to be taken. This second edition reasserts the book's uniqueness in its detailed discussion of the topic, making it required reading for all those working within the field of peritoneal dialysis.
This new clinical resource brings you a state-of-the-art comprehensive review on every clinical condition encountered in pediatric nephrology in one concise, clinically focused text. International experts provide you with the latest on epidemiology, diagnosis, investigations, management, and prognosis for a full range of pediatric kidney disorders. A full-color, highly visual, meticulously crafted format, makes this material remarkably easy for you to access and apply. Comprehensive Pediatric Nephrology also serves as an ideal resource for board review study for the ABP subspecialty boards in pediatric nephrology. Just the right amount of "need-to-know" basic science coupled with practical c...
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This book surveys experiences made with Polymethylmethacrylate (PMMA) dialysis membranes, ranging from a summary of the most recent experimental and clinical results to an overview of future potentials and new trends in research. Several articles treat the biophysical properties of the membrane, its chemical structure and manufacturing procedures aimed at obtaining hollow fibers suitable for medical purposes. Moreover, various experimental conditions are considered in order to identify the dialytic properties of the PMMA membrane and dialyzer series. In this context, particular emphasis is put on the last generation of PMMA membranes with higher porosity and ultrafiltration capacity. On the ...