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A range of novel techniques is available to the plant breeder today to complement classical breeding methods. The new options are based on the integration of advances in plant cell biology with those in plant molecular biology. Plant cell, tissue and organ cultures provide efficient systems for transformation, for the achievement of wide crosses and for the production of variation through spontaneous and induced mutation, while permitting effective isolation of desired genotypes by in vitro selection. This book presents a critical appraisal of the methodologies of plant genetic manipulation for advanced undergraduates, postgraduates, researchers and plant breeders, and provides guidance on the choice of breeding options. The latter depends on the breeding system of the crop, the breeding objective and the tissue culture systems applicable to the target genotype(s).
Humanity is facing many global challenges. These include 1) achieving food security for a rapidly growing population, 2) slowing the progression of climate change by reducing the production and release of greenhouse gases as consequence of human activity, and 3) meeting the increasing demand for clean energy that will not harm the environment. In this regard, legumes deliver several important services to societies. Legumes provide a diverse range of food crops that are significant sources of plant-based proteins for humans globally. Grain legumes present outstanding nutritional and nutraceutical properties, while being an affordable food that contributes to achieving future global food and feed security in the context of an increasing world population.
The Editorial Office of Frontiers in Plant Science would like to thank all the Chief Editors, Associate Editors and Review Editors that played an integral part in Frontiers’ innovative Collaborative Peer-Review process in 2020. In particular, we would like to recognize and thank Prof. Joshua L. Heazlewood – our now former Field Chief Editor, for his commitment, support and enthusiasm for the Plant Science field. Josh’s dedication and leadership has helped Frontiers in Plant Science become the most cited journal in the field with a strong editorial community. Looking forward, we’re excited to welcome Prof. Yunde Zhao, as our new Field Chief Editor in 2021. Having been with Frontiers in Plant Science since 2017, Yunde has contributed extensively to the development of the journal and will continue to ensure the journal goes from strength to strength.
This fifth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniques, and laborious process of measuring the epigenetics changes in tissue cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Cell Culture Protocols, Fifth Edition aims to ensure successful results in the further study of this vital field.
Provides a systematic review of the latest techniques of biotechnology as applied to perennial fruit crops. The book emphasizes genetic manipulation rather than tissue culture or micropropagation. Individual fruit crops are covered as well as methodologies applicable to a range of fruits.
Today it is generally accepted that one of the key areas of biotechnology for the next century will be in plant-based biotechnology. Biotechnology has created new opportunities for plant scientists, with important applications to agriculture and forestry. This reference text is divided into five sections for ease of presentation. The first section focuses on the structure, composition and functionality of plant cells and genes with particular emphasis on the cellular and molecular biology of plants and cultured cells. Section two is concerned with the direct exploitation of cell cultures for the production of useful substances. The third section deals with regeneration and propagation systems. The fourth section considers the increasingly central area of genetic manipulation of plant cell systems. The last section is on specific applications in plant biotechnology. This reference work is a survey of these various facets of plant biotechnology. The individual chapters and the follow-up literature cited allow an easy access to the various subject areas and will, hopefully, stimulate interest in these rapidly moving and exciting fields of research.
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