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Regulators and Effectors of Small GTPases, Part D: Rho Family
  • Language: en
  • Pages: 583

Regulators and Effectors of Small GTPases, Part D: Rho Family

  • Type: Book
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  • Published: 2000-10-11
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  • Publisher: Elsevier

This volume and its companions (Volumes 255, 256, 257, and the forthcoming 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Combinatorial Chemistry, Part B
  • Language: en
  • Pages: 607

Combinatorial Chemistry, Part B

  • Type: Book
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  • Published: 2004-01-26
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  • Publisher: Elsevier

Combinatorial Chemistry encompasses both the design of compounds for specific pharmacological use and the screening of molecules in high throughput automated tests to find active agents with specific functions.*Analytical techniques*Direct sorting split and pool combinatorial synthesis*Linkers and their applications*Microwave assisted synthesis*Oligosaccharide chemistry*Peptide Synthesis and Screening*Polymer assisted approaches*Small molecule and heterocycle synthesis

Protein Sensors and Reactive Oxygen Species, Part B: Thiol Enzymes and Proteins
  • Language: en
  • Pages: 439

Protein Sensors and Reactive Oxygen Species, Part B: Thiol Enzymes and Proteins

  • Type: Book
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  • Published: 2002-03-06
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  • Publisher: Elsevier

This volume of Methods in Enzymology is a companion to Volume 347 and addresses direct sensing of reactive oxygen species and related free radicals by thiol enzymes and proteins.

Sphingolipid Metabolism and Cell Signaling
  • Language: en
  • Pages: 688

Sphingolipid Metabolism and Cell Signaling

This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Vertebrae Phototransduction and the Visual Cycle, Part B
  • Language: en
  • Pages: 887

Vertebrae Phototransduction and the Visual Cycle, Part B

  • Type: Book
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  • Published: 2000-04-19
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  • Publisher: Elsevier

Major topics covered include photoreceptor proteins, phototransduction calcium-binding proteins and calcium measurement in photoreceptor cells, enzymes of the visual cycle, posttranslational and chemical modifications, analysis of animal models of retinal diseases. Inherited retinal disease; from the defective gene to its function and repair. This volume and its companion Volume 315 include newly developed methods to study vertebrate phototransduction and the visual cycle.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Guide to Yeast Genetics and Molecular and Cell Biology, Part C
  • Language: en
  • Pages: 782

Guide to Yeast Genetics and Molecular and Cell Biology, Part C

This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology.

Nitric Oxide, Part D
  • Language: en
  • Pages: 553

Nitric Oxide, Part D

  • Type: Book
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  • Published: 2002-12-09
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  • Publisher: Elsevier

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. - Presents information on nitrosothiols and nitric oxide in cell signaling - Details nitric oxide and mitochondrial functions - Explains nitric oxide synthases

Oxygen Radicals in Biological Systems
  • Language: en
  • Pages: 758

Oxygen Radicals in Biological Systems

  • Type: Book
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  • Published: 1994
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  • Publisher: Elsevier

Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 234 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. Key Features* Generation, detection, and characterization of oxygen radicals, chemistry, biochemistry, and intermediate states of reductio* Isolation, characterization, and assay of enzymes or substrates involved in formation or removal of oxygen radical* Methods for assessing molecular, cell, and tissue damage; assays and repair of oxidative damage.

Nuclear Magnetic Resonance of Biological Macromolecules, Part C
  • Language: en
  • Pages: 670

Nuclear Magnetic Resonance of Biological Macromolecules, Part C

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Nuclear Magnetic Resonance of Biological Macromolecules, Part C is written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules.* One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences

Redox Cell Biology and Genetics, Part A
  • Language: en
  • Pages: 617

Redox Cell Biology and Genetics, Part A

  • Type: Book
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  • Published: 2002-07-22
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  • Publisher: Elsevier

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.Key Features* Cellular Responses* Tissues and Organs