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Flow Cytometry
Advances in Cytometry: Applications, Volume 176 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the topic of development, characterization and applications in CAR T Cells
In recent years new discoveries have made this an exciting and important field of research. This exhaustive volume presents comprehensive chapters and detailed background information for researchers working with in the field of nuclear mechanics and genome regulation. - Both classic and state-of-the-art methods readily adaptable and designed to last the test of time - Relevant to clinicians and scientists working in a wide range of fields
Genetic Expression in the Cell Cycle provides an understanding of the molecular mechanisms that govern the expression of genetic information during the cell cycle. The initial five chapters describe the intimate relationships between the supramolecular complexes that form the basic structure of chromatin. Emphasis is placed on the dynamics of cycle-dependent changes in the structural organization of some of these components. Subsequent chapters demonstrate that small nuclear RNAs (SnRNA) are actively involved in gene regulation in eukaryotic cells; discuss the relationship between cell cycle regulation in the yeast Saccharomyces cerevisiae and transcription of ribosomal RNA genes; and describe the use of conditional lethal mutants to study the regulation of the cell cycle of eukaryotic cells. The remaining chapters discuss the concepts and methodologies employed to isolate and study specific cell cycle mutants of S. cerevisiae; the antiproliferative effect of interferon on cultured human fibroblasts; and the role of cell membrane and related subcellular elements in the control of proliferation, differentiation, and cell cycle kinetics.
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users.
Apoptosis provides a current and comprehensive collection of methods for the study of cell death. Using a diverse range of technical approaches and model systems, the chapters in this volume cover topics from the cellular and organismal to the molecular and anatomical. The methods are illustrated with user-friendly recipes and over 100 tables, halftones, and diagrams. - Current methodologies for studying cell death - Wide range of model systems - Molecular, biochemical, cellular, and genetic approaches - Complements the original Cell Death volume - Up-to-date methodology for a fast moving field - Designed with the needs of both basic scientists and clinicians in mind - Authors are leaders in their respective fields
V. 1: cell and tissue culture and associated techniques; Primary cultures from embyonic and newborn tissues; Culture of specific cell types; Cell separation techniques; Model systems to study differentiation; cell cycle analysis; Assays of tumorigenicity, invasion, and others; Cytotoxic and cell growth assays; Senescence and apoptosis; Electrophysiological methods; Histocultures and organ cultures; Other cell types and organisms; Viruses; Appendices; v. 2: Organelles and cellular structures; Assays; Antibodies; Immunocytochemistry; Vital staining of cells; v. 3: Light microscopy and contrast generation; Electron microscopy; Intracellular measurments; Cytogenetics and in situ hybridization; transgenic and gene knockouts; v. 4: Transfer of macromelcules and small molecules; Expression systems; Differential gene expression; Proteins; Appendix; List of suppliers; Subject index.
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Alphabetical listing of manuscript instructions to over 500 medical and scientific journals. Also contains a list of journal titles by subject.