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This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase–seq for identifying nucleosome occupancy, alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.
Modern agriculture needs to review and broaden its practices and business models, by integrating opportunities coming from different adjacent sectors and value chains, including the bio-based industry, in a fully circular economy strategy. Searching for new tools and technologies to increase crop productivity under optimal and sub-optimal conditions and to improve resources use efficiency is crucial to ensure food security while preserving soil quality, microbial biodiversity, and providing business opportunities for farmers. Biostimulants based on microorganisms or organic substances obtained from renewable materials represent a sustainable, efficient technology or complement to synthetic c...
Sandler contends that the impact of the nation in foreign policy is not synonymous with that of the State. Understanding the effect of the nation is important because of the contemporary reawakening of primordial national aspirations. This study is designed to test these views by examining nation-centered concerns in foreign policy as practiced within Israel. It reviews and analyzes the roots of the territorial dimension in Israeli foreign policy since the establishment of the state up to the present; the impact of Israeli domestic politics; and the rise and fall of ethnonationalism in Israeli foreign policy. As such, the work is of concern to all students of Israeli politics and foreign policy and the Arab-Israeli conflict.
The goal of this book is to treat Palestine not as a state but as a country which in 1948 was divided to Israel, The West Bank, and the Gaza Strip. Palestinians live in all these areas and are also dwellers or refugee camps and exilic communities around the world. In our eyes they and the country as a whole are part of the history of Palestine and therefore are all included here. It is a book that regards Palestine in the period from 1800 until today as a geographical term which is still valid and relevant. Therefore, it covers different geo-political units and states that were established over the year in the country of Palestine: the late Ottoman provinces, the British Mandate, the State I...
Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the “omics” tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Arabidopsis thaliana is acknowledged as the most important plant model syste...