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Extremely halophilic archaea (salt-loving archaea), termed haloarchaea, constitute one of the largest groups within the Archaea and inhabit the most hypersaline environments on Earth. Due to their easy cultivation in the laboratory and rapid growth kinetics, haloarchaea are excellent models for studying cell biology of Archaea. Research into haloarchaea have enabled tremendous progress in various areas of archaeal biology including genetic evolution, gene expression and regulation, protein synthesis and adaptation, structural dynamics, and various biotechnological applications. The detailed investigation of survival and adaptation mechanisms of haloarchaea to high salt environments have provided key insights into the boundaries of life, improving our knowledge on life evolution on Earth and expanding the realms possible for the search for extraterrestrial life.
This book presents the latest results in the exploration of halophilic bacteria, archaea, fungi and viruses. Basic and molecular aspects as well as possible biotechnological applications of halophiles are highlighted by leading scientists. Topics include: the family Halomonadaceae; the hypersaline lakes of Inner Mongolia ; Salinibacter ruber - from genomics to microevolution and ecology; the impact of lipidomics on the microbial world of hypersaline environments; molecular mechanisms of adaptation to high salt concentration in the black yeast Hortaea werneckii; viruses in hypersaline environments; initiation and regulation of translation in halophilic Archaea; protein transport into and across haloarchaeal cytoplasmic membranes; protein glycosylation in Haloferax volcanii; the effect of anoxic conditions and temperature on gas vesicle formation in Halobacterium salinarum; halophiles exposed to multiple stressors; cellular adjustments of Bacillus subtilis to fluctuating salinities; the nature and function of carotenoids in Halobacillus halophilus; xanthorhodopsin; enzymatic biomass degradation by halophilic microorganisms; and enzymes from halophilic Archaea.
This volume provides an overview of well-established methods optimized for diverse archaeal model organisms and is a source of protocols facilitating access to the molecular and cellular biology characterization of these fascinating organisms. Chapters are divided into five parts detailing available genetic tools, molecular and cellular biology methods, strategies to study the ecophysiology of archaea, and classroom protocol. Each main thematic part is also introduced by future-oriented and authoritative primers. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Archaea: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
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ELF researchers have been describing the dynamic and fluid ways in which multilingual speakers shape English in transcultural communication for more than two decades now. While this work seriously challenges traditional, static, and prejudiced views of English, the diverse and variable nature of its uses and users continues to be undermined in many EFL programs around the world. This is also the case in many Latin American contexts, which have been described as fertile ground for native-speaker ideology, but where the body of ELF literature is still scarce when compared to Asian and European settings. This book is the first to bring together a series of empirical studies on the implications ...
Clippings of Latin American political, social and economic news from various English language newspapers.
Vols. for 1905?-19 include Sección oficial, separately paged.
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