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Many girls want to become scientists when they grow up, just like many boys do. But for these girls, the struggle to do what they love and to be treated with respect has been much harder because of the discrimination and bias in our society. In Women in Microbiology, we meet women who, despite these obstacles and against tough odds, have become scientific leaders and revered mentors. The women profiled in this collection range from historic figures like Alice Catherine Evans and Ruth Ella Moore to modern heroes like Michele Swanson and Katrina Forest. What binds all of these remarkable women are a passion for their work, a zest for life, a warm devotion to mentoring others—especially young...
Since their identification four decades ago, Archaea have proven to be a continuous source of exciting discoveries, contributing to the characterization of their unique molecular mechanisms, metabolisms, phylogeny, and cell biology. These discoveries have revealed the importance that Archaea play in ecology, biotechnology and the human microbiome. In addition, they highlighted the key position that Archaea occupy in the tree of life, bringing us closer to elucidating the origin and early forms of life. Despite these important findings and the larger audience that Archaea have consequently gained, much remains unexplored. Thanks to the recent and ongoing developments in the field, technical limitations at the often-extreme archaeal growth conditions are being resolved, allowing archaeal researchers to answer open and upcoming questions. This promises exciting new findings in the near future that will continue to build on our understanding of the various fields of archaeal biology.
Extremely halophilic archaea (salt-loving archaea), termed haloarchaea, constitute one of the largest groups within the Archaea and inhabit the most hypersaline environments on Earth. Due to their easy cultivation in the laboratory and rapid growth kinetics, haloarchaea are excellent models for studying cell biology of Archaea. Research into haloarchaea have enabled tremendous progress in various areas of archaeal biology including genetic evolution, gene expression and regulation, protein synthesis and adaptation, structural dynamics, and various biotechnological applications. The detailed investigation of survival and adaptation mechanisms of haloarchaea to high salt environments have provided key insights into the boundaries of life, improving our knowledge on life evolution on Earth and expanding the realms possible for the search for extraterrestrial life.
The accumulation of archaeal genomes has lagged significantly behind the Bacteria; however, in the last several years the coverage of the major phyla of Archaea has been significantly improved. There are now multiple genomes in several important genera such as Pyrobaculum, Sulfolobus, Thermococcus/Pyrococcus, Halobacterium, Methanosarcina, Methanopyrus and Methanocaldococcus. Comparative genomic studies are now under way, and in many cases there are several consortial multilaboratory groups, such as the SulfoSys community, which have started to break into new systems biology initiatives. At the same time, access to streamlined genetic approaches in the genera Sulfolobus, Thermococcus, Methan...
This volume provides an overview of well-established methods optimized for diverse archaeal model organisms and is a source of protocols facilitating access to the molecular and cellular biology characterization of these fascinating organisms. Chapters are divided into five parts detailing available genetic tools, molecular and cellular biology methods, strategies to study the ecophysiology of archaea, and classroom protocol. Each main thematic part is also introduced by future-oriented and authoritative primers. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Archaea: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
We might think of them as living on the very edge of existence. Referred to as extremophiles, these microorganisms exhibit the most radical capacity for adaptation in those harsh environments that are just barely conducive to the existence of cellular life. Unlocking the mechanisms and understanding the evolutionary development that allows these si
Archaea represent a third domain of life with unique properties not found in the other domains. Archaea actively compete for environmental resources. They perceive themselves and can distinguish between ‘self’ and ‘non-self’. They process and evaluate available information and then modify their behaviour accordingly. They assess their surroundings, estimate how much energy they need for particular goals, and then realize the optimum variant. These highly diverse competences show us that this is possible owing to sign(aling)- mediated communication processes within archaeal cells (intra-organismic), between the same, related and different archaeal species (interorganismic), and betwee...
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Archaea and Bacteria have complex cell envelopes that play important roles in several vital cellular processes, including serving as a barrier that protects the cytoplasm from the environment. Along with associated proteinaceous structures, cell envelopes also ensure cell stability, promote motility, mediate adherence to biotic and abiotic surfaces, and facilitate communication with the extracellular environment. While some aspects of the biosynthesis and structure of the cell are similar to the three domains of life, archaeal cell envelopes exhibit several unique characteristics. Moreover, recent analyzes have revealed that many features of cell envelopes can vary greatly between distantly ...
*Extremophiles: Overview of the Biotopes *Phylogeny of Extremophiles *Survival Strategies and Membrane Properties of Bacteria and Archaea in Extreme Environments